Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood.
2.
Supravital staining can be combined with cell surface antibody staining ( immunofluorescence ) for applications such as FACS analysis.
3.
A traditional application of methylene blue is the intravital or supravital staining of nerve fibers, an effect first described by Paul Ehrlich in 1887.
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